Core technologies and resources

The Marasco Laboratory’s core technologies include human antibody discovery and engineering through phage, yeast, and mammalian antibody display libraries, antibody affinity maturation and humanizatrion with structure modeling and recombinat DNA techniques, and development antibody therapeutics against infectious disease and cancer including vaccine studies utilizing several human immune system reconsitited mouse models. The lab is equipped with state-of-the-art equipment such as Biacore T100, OctetRed96, MSD (Meso Scale Discorvery) Sector Imager 2400, PolarStar Omega Multilabel Plate Reader, AktaExplorer FPLC, and FACSCalibor. In addition, our newly acquired robotic system including RapidLite Colony Picker and Tecan Freedom Evo 200 Liquid Handler with integrated ELISA reader, power washers and two incubators allow the lab to perform automated high throughput antibody discovery.

Twenty Core Facilities at DFCI provide expertise and services to support the lab’s research:

Animal Facility
Biohazard Containment Suite (BL2+ with BL3 practices)
Biospecimen Repository
Biostatistics & Computational Biology
Center for Cancer Computational Biology
Clinical Research Laboratory
Confocal and Light Microscopy
Connell-O’Reilly/Cell Manipulation Core
Critical Alarm Monitoring System
Survey and Data Management
Flow Cytometry
Health Communication
Medical Arts
Medicinal Chemistry
Molecular Biology Core Facilities
Molecular Diagnostics Laboratory
Monoclonal Antibody
RNA Interference (RNAi) Screening Facility
Tumor Imaging Metrics

Located in the center of the Longwood Medical Area, facilities and cores of Harvard Medical School and its other affiliated hospitals are also accessible to the Marasco Laboratory.  Of particular interest are the pathology cores, the BL3 facilities at the New England Regional Center of Excellence for Biodefense and Emerging Infectious Diseases (NERCE/BEID), and the New England Primate Research Center (NEPRC), to name a few.