Antibody engineering

We are leaders in the field of human antibody engineering and have been involved in many of the seminal discoveries in the field. We are experts in phage, yeast and lentivirus display platforms and panning procedures, human antibody library constructions, Fc engineering, humanization procedures and targeted siRNA delivery to name a few. We have constructed human antibody libraries with tens of billion members and have successfully isolated therapeutic human antibodies against over two dozen targets. We have also developed and utilize antibody expression systems in bacteria, mammalian, yeast and insect cells. Students and post-doctoral fellows join the Marasco lab to become skilled artisans in the field under Dr. Marasco’s mentorship.For an overview of some of our antibody engineering tools can be found in the following link.

Gennari F, Lopes L, Verhoeyen E, Marasco WA and Collins MK. Single-chain antibodies that target lentiviral vectors to MHC Class II on antigen-presenting cells. Gene Therapy. Gene Therapy, 2009 Jun;20(6):554-62. PMID: 19260768

Taube R, Zhu Q, Xu C, Diaz-Griffero F, Sui J, Aird D, Dwyer MR and Marasco WA. Lentivirus Display: Stable Expression of Human Antibodies on the Surface of Human Cells and Virus Particles. PLoS ONE. 2008 Sep 11;3(9):e3181. PMID: 18784843 PMCID: PMC2527531

Marasco WA and Sui J. The growth and potential of human antiviral monoclonal antibody therapeutics. Nat Biotechnol. 2007 1421-34. PMID: 18066039

Xu C, Sui J, Tao H, Aird D, Zhu QK and Marasco WA. Human Anti-CXCR4 Antibodies Undergo VH Replacement, Exhibit Functional V-Region Sulfation and Define CXCR4 Antigenic Heterogeneity. J. Immunol 2007 Aug 15;179(4):2408-18. PMID: 17675502

Bal HP, Cheng J, Murakami A, Tallarico ASC, Wang W, Zhou D, Vasicek TJ and Marasco WA. Detection of GITR overexpression in HTLV-1 transformed cells by massively parallel signature sequencing. Biochem. Biophys. Res. Commun. 2005 Jul 1;332(2):569-84. PMID: 15896717

Marasco WA. Therapeutic antibody gene transfer. Nat Biotechnol. 2005 May;23(5):551-2. PMID: 15877069

Beyer F, Doebis C, Busch A, Ritter T, Mhashilkar A, Marasco WA, Laube H, Volk HD, Seifert M. Decline of Surface MHC I by Adenoviral Gene Transfer of Anti-MHC I Intrabodies in Human Endothelial Cells - New Perspectives for the Generation of Universal Donor Cells for Tissue Transplantation. J Gene Med. 2004 Jun;6(6):616-23. PMID: 15170732

Zhu Q, Ricardo RR, Zhang L, Ogueta SB, Agrawal RS, Dzau VJ, and Marasco WA. Development of constitutive and inducible self-inactivating lentiviral vectors and their application in cardiovascular gene transfer. Gene Ther Mol Biol., 2004; (8): 91-102. pdf

Gennari F, Mehta S, Wang Y, Tallarico A, Palu G, and Marasco WA. Direct Phage to Intrabody Screening: Demonstration by Isolation of Cytosolic Intrabodies Against the TES1 Site of Epstein Barr Virus Latent Membrane Protein 1 (LMP1) that Block NF-kB Transactivation. Journal of Molecular Biology, 2004; 335: 193 - 207. PMID: 14659750

Busch, A, Marasco, WA, Doebis, C, Volk, H-D, Seifert, M. MHC Class I Manipulation on Cell Surfaces by Gene Transfer of Anti-MHC Class I Intrabodies – A tool for Decreased Immunogenicity of allogeneic tissue and cell transplants. Methods, 2004; 34(2):240-249. PMID: 15312677

Sui J, Bai J, St Clair Tallarico A, Xu C, Marasco WA. Identification of CD4 and transferrin receptor antibodies by CXCR4 antibody-guided Pathfinder selection. Eur J Biochem 2003 Nov;270:4497-506. PMID: 14622278

Mhashilkar AM, Doebis C, Seifert M, Busch A, Zani C, Soo Hoo J, Nagy M, Ritter T, Hans-Dieter V, and Marasco WA. Intrabody-Mediated Phenotypic Knockout of Major Histocompatibility Complex Class 1 Expression in Human and Monkey Cell Lines and in Primary Human Keratinocytes. Gene Ther 2002;9:307-319. PMID: 11938450