Publications and presentations

Keynote Presentations


The Basis and Bias of Human Anti-Influenza Neutralizing Antibody Responses
IBC's 26th Antibody Engineering & Therapeutics Conference. San Diego, CA


What Questions Haven’t Been Addressed with Respect to Stem Cells, Cancer and Aging?
The Vatican. Rome, Italy

Studies Toward Developing an Universal Influenza Vaccine in Man
Translational Research Seminar Series. Washington Univ., St. Louis, MO


Immunologic Challenges to the Development of a Universal Influenza Vaccine
NIH Influenza Conference. Bethesda, MD

Human Anti-CCR4 mAb Immunotherapy for the Treatment of Cutaneous T-cell Lymphoma
PEGS Conference. Boston, MA


Adult Stem Cells: Science and the Future of Man and Culture
The Vatican. Rome, Italy

The Basis and Bias of Broad Spectrum Human Anti-Influenza Antibody. Human & Translational Immunology Series Seminar
Yale University School of Medicine. New Haven, CT


The Basis and Bias of Human Anti-Influenza Antibody Responses
Gordon Research Conferences: Antibody Biology & Engineering - From Basic Mechanisms To Antibody-Based Therapeutics. Ventura, CA

Broad-Spectrum Neutralizing Antibodies to Influenza A Viruses.
Genentech. San Francisco, CA


The Potential of Stem Cell Treatment of Human Disease.
Long Island Jewish Hospital. Grand Rounds. Manhasset, NY

The Basis and Bias of the Human Anti-Viral Antibody Response.
Walter Reed Army Institute of Research. Distinguished Speaker.
Silver Spring, MD

Structural and Functional Bases for Broad-Spectrum Neutralization of Avian and Human Influenza A Viruses.
Novartis. Cambridge, MA

Full List of Publications

Fu Y, Zhang Z, Sheehan J, Avnir Y, Ridenour C, Sachnik T, Sun J, Hossain MJ, Chen LM, Zhu Q, Donis RO, Marasco WA. A broadly neutralizing anti-influenza antibody reveals ongoing capacity of haemagglutinin-specific memory B cells to evolve. Nat Commun. Accepted

Suarez ER, Chang DK, Sun J, Sui J, Freeman GJ, Signoretti S, Zhu Q, Marasco WA. Chimeric antigen receptor T cells secreting anti-PD-L1 antibodies more effectively regress renal cell carcinoma in a humanized mouse model. Oncotarget. 2016 Apr 29. doi: 10.18632/oncotarget.9114. PMID: 27145284.

Menachery VD, Yount BL Jr, Sims AC, Debbink K, Agnihothram SS, Gralinski LE, Graham RL, Scobey T, Plante JA, Royal SR, Swanstrom J, Sheahan TP, Pickles RJ, Corti D, Randell SH, Lanzavecchia A, Marasco WA, Baric RS. SARS-like WIV1-CoV poised for human emergence. Proc Natl Acad Sci U S A. 2016 Mar 14. PMID: 26976607.  

Chang DK, Kurella VB, Biswas S, Avnir Y, Sui J, Wang X, Sun J, Wang Y, Panditrao M, Peterson E, Tallarico A, Fernandes S, Goodall M, Zhu Q, Brown JR, Jefferis R, Marasco WA. Humanized mouse G6 anti-idiotypic monoclonal antibody has therapeutic potential against IGHV1-69 germline gene-based B-CLL. MAbs. 2016 Mar 10:1-12. PMID: 26963739. 

Avnir Y, Watson CT, Glanville J, Peterson EC, Tallarico AS, Bennett AS, Qin K, Fu Y, Huang CY, Beigel JH, Breden F, Zhu Q, Marasco WA. IGHV1-69 polymorphism modulates anti-influenza antibody repertoires, correlates with IGHV utilization shifts and varies by ethnicity. Sci Rep. 2016 Feb 16;6:20842. PMID: 26880249. PMCID: PMC4754645. 

Johnson RF, Bagci U, Keith L, Tang X, Mollura DJ, Zeitlin L, Qin J, Huzella L, Bartos CJ, Bohorova N, Bohorov O, Goodman C, Kim do H, Paulty MH, Velasco J, Whaley KJ, Johnson JC, Pettitt J, Ork BL, Solomon J, Oberlander N, Zhu Q, Sun J, Holbrook MR, Olinger GG, Baric RS, Hensley LE, Jahrling PB, Marasco WA. 3B11-N, a monoclonal antibody against MERS-CoV, reduces lung pathology in rhesus monkeys following intratracheal inoculation of MERS-CoV Jordan-n3/2012. Virology. 2016 Mar;490:49-58. PMID: 26828465. PMBID: PMC4769911. 

Menachery V, Yount BL, Debbink K, Agnihothram S, Gralinski L, Plante JA, Graham R, Scobey T, Donaldson EF, Ge XY, Randell SH, Lanzavecchia A, Marasco WA, Shi ZL, Baric R. A SARS-like cluster of circulating bat coronaviruses pose threat for human emergence. Nat Med. 2015 Dec;21(12):1508-13. PMID: 26552008. PMCID: PMC4797993. 

Freund N, Roitburd-Berman A, Sui J, Marasco WA, Gershoni JM. Reconstitution of the Receptor Binding Motif of the SARS Coronavirus. Protein Eng Des Sel. 2015 Dec;28(12):567-75. PMID: 26487711. 

Chang DK, Peterson E, Sun J, Goudie C, Drapkin RI, Liu JF, Matulonis U, Zhu Q and Marasco WA. Anti-CCR4 antibody enhances anti-tumor immunity by modulating tumor-infiltrating Tregs in an ovarian cancer xenograft humanized mouse model. OncoImmunol. 2015 Dec 10;5(3):e1090075. 

Tang XC, Marasco WA. Human neutralizing antibodies against MERS coronavirus: implications for future immunotherapy. Immunotherapy. 2015 Jul;7(6):591-4. PMID: 26098703. 

Chang DK, Moniz RJ, Xu Z, Sun J, Signoretti S, Zhu Q, Marasco WA. Human anti-CAIX antibodies mediate immune cell inhibition of renal cell carcinoma in vitro and in a humanized mouse model in vivo. Mol Cancer. 2015 Jun 11;14:119. PMID: 26062742.  

Huang X, Dong W, Milewska A, Golda A, Qi Y, Zhu QK, Marasco WA, Baric RS, Sims AC, Pyrc K, Li W, Sui J.  HCoV-HKU1 Spike protein uses O-acetylated sialic acid as an attachment receptor determinant and employs HE protein as a receptor-destroying enzyme.  J Virol. 2015 Apr 29. pii: JVI.00854-15.  PMID: 25926653

Goettel JA, Biswas S, Lexmond WS, Yeste A, Passerini L, Patel B, Yang S, Sun J, Ouahed J, Shouval DS, McCann KJ, Horiwitz BH, Mathis D, Milford EL, Notarangelo LD, Roncarolo MG, Fiebiger E, Marasco WA, Bacchetta R, Quintana FJ, Pai SY, Muise AM, Snapper SB.  Fatal autoimmunity in mice reconstituted with human hematopoietic stem cells encoding defective FOXP3.  Blood. 2015 Apr 1. pii: blood-2014-12-618363.  PMID: 25833964

Kang W, Marasco WA, Tong HI, Byron MM, Wu C, Shi Y, Sun S, Sun Y, Lu Y.  Anti-tat Hutat2: Fc mediated protection against tat-induced neurotoxicity and HIV-1 replication in human monocyte-derived macrophages. J Neuroinflammation. 2014 Nov 22;11(1):195. doi: 10.1186/s12974-014-0195-2.  PMID: 25416164

Sui J, Deming M, Rockx B, Liddington RC, Zhu QK, Baris RS, Marasco WA.  Effects of human anti-spike protein receptor binding domain antibodies on severe acute respiratory syndrome coronavirus neutralization escape and fitness.  J Virol. 2014 Dec; 88(23):13769-80. doi: 10.1128/JVI.02232-14. Epub 2014 Sep 17.  PMID:25231316

Abdel-Motal UM,  Harbison C, Han T, Pudney J, Anderson DJ, Zhu Q, Westmoreland S, Marasco WA.  Prolonged expression of an anti-HIV-1 gp120 minibody to the female rhesus macaque lower genital tract by AAV gene transfer.  Gene Therapy. June 26, 2014; doi: 10.1038/gt.2014.56. pdf.

Avnir Y, Tallarico AS, Zhu Q, Bennett AS, Connelly G, Sheehan J, Sui J, Fahmy A, Huang C-Y, Cadwell G, Bankston LA, McGuire AT, Stamatatos L, Wagner G, Liddington RC, Marasco WA.  Molecular Signatures of Hemagglutinin Stem-Directed Heterosubtypic Human Neutralizing Antibodies Against Influenza A Viruses. PLOS Pathogens. May 1, 2014, Vol 10, Issue 5 DOI:10.1371 PMID: 24788925 pdf

Tang XC, Agnihothram SS,Jiao Y, Stanhope J, Graham RL, Peterson EC, Avnir Y, Tallarico AS,Sheehan J, Zhu Q, Baric RS, Marasco WA.  Identification of human neutralizing antibodies against MERS-CoV and their role in virus adaptive evolution. PNAS April 28, 2014  PMID: 24778221 pdf

Hall SR, Jiang Y, Leary E, Yavanian G, Eminli S, O'Neill DW, Marasco WA.  Identification and Isolation of Small CD44-Negative Mesenchymal Stem/Progenitor Cells From Human Bone Marrow Using Elutriation and Polychromatic Flow Cytometry.  Stem Cells Transl Med.  2013 Aug;2(8):567-78. doi:10.5966/sctm.2012-0155. Epub 2013 Jul 11.  PMID:2384700

Zhang J, Valianou M, Simmons H, Robinson MK, Lee HO, Mullins SR, Marasco WA, Adams GP, Weiner LM, Cheng JD.  Identification of inhibitiry scFv antibodies targeting fibroblast activation protein utilizing phage display functional screens.  FASEB J.  2013 Feb;27(2):581-9. doi:10.1096/fl. 12-210377. Epub 2012 Oct 26.  PMID: 23104982

Han T, Abdel-Motal UM, Chang DK, Sui J, Muvaffak A, Campbell J, Zhu Q, Kupper TS, Marasco WA.  Human Anti-CCR4 Minibody Gene Transfer for the Treatment of Cutaneous T-Cell Lymphoma.  PLoS ONE Sep 4 2012 | PMID:22973452 | PMCID:PMC3433438

Vyas JM, Marasco WA.  Fatal fulminant hepatic failure from adenovirus in allogeneic bone marrow transplant patients. Case Rep Infect Dis. 2012;2012:463569.  Epub 2012 Aug 15.  PMID: 22928129 PMCID: PMC3426185

Chang DK, Sui J, Geng S, Muvaffak A, Bai M, Fuhlbrigge RC, Lo AS, Yammanuru A, Hubbard L, Sheehan J, Campbell JJ, Zhu Q, Kupper TS and Marasco WA.  Humanization of an anti-CCR4 antibody that kills Cutaneous T Cell Lymphoma cells and abrogates suppression by T regulatory cells.  Mol Cancer Ther.  2012 Aug 6.  PMID: 22869555  PMCID:PMC3496034

Hammond SP, Gange LS, Stock SR, Marty FM, Gelman RS, Marasco WA, Poritz MA, Baden LR.  Respiratory Virus Detection with FilmArray Respiratory Panel Compared to Conventional Methods in Immunocompromised Patients.  J Clin Microbiol. 2012 Jul 18.  PMID: 22814461

Braun SE, Taube R, Zhu Q, Wong FE, Murakami A, Kamau E, Dweyer M, QIU G, Daigle J, Carville A, Johnson RP and Marasco WA.  In Vivo Selection of CD4+ T Cells Transduced with a Gamma-Retroviral Vector Expressing a Single-Chain Intrabody Targeting HIV-1 Tat.  Hum Gene Ther.  2012 Jun 26.  PMID: 22734618 

Chang H, Biswas S, Tallarico AS, Sarkis P, Geng S, Panditrao MM, Zhu Q, Marasco WA.  Human B-cell ontogeny in humanized NOD/SCID gamma_c_null mice generates a diverse yet auto/poly- and HIV-1 reactive antibody repertoire.  Genes and Immunity. 13, 399-410 (July/August 2012) | doi:10.1038/gene.2012.16.  PMID:22592523.

Abdel-Motal U, Sarkis PTN, Han T, Pudney J, Anderson DJ, Zhu Q, Marasco WA.  Anti-gp120 minibody gene transfer to female genital epithelial cells protects against HIV-1 virus challenge in vitro. PLoS One. 2011, 2011;6(10):e26473. PMCID: PMC3198777.  

Biswas S, Chang H, Sarkis PTN, Fikrig E , Zhu Q and Marasco WA.  Humoral immune responses in humanized BLT mice immunized with West Nile virus and HIV-1 envelope proteins are largely mediated via human CD5+ B cells.  Immunology, 2011, Dec;134(4):419-33. PMID: 22044090.

Han T, Sui J, Bennett AS, Liddington RC, Donis RO, Zhu Q and Marasco WA. Fine epitope mapping of monoclonal antibodies against hemagglutinin of a highly pathogenic H5N1 influenza virus using yeast surface display Biochem Biophys Res Commun. 2011; Jun 3; 409(2):253-9. Epub 2011 May5. PMID: 21569761. PMCID:PMC3119598.

Sui J, Sheehan J, Hwang WC, Bankston, LA, Burchett SK, Huang CY, Liddington R, Beigel JH, and Marasco WA. Wide Prevalence of Heterosubtypic Broadly Neutralizing Human Anti-Influenza A Antibodies. Clinical Infectious Diseases. 2011 Apr 15;52(8):1003-9; PMID: 21460314.

Han T and Marasco, WA. Structural basis of influenza virus neutralization.  Ann N Y Acad Sci. The Year in Immunology. 2011 Jan;1217(1):178-90. PMID: 21251008; PMCID:PMC3062959.

Wrammert J, Koutsonanos D, Li GM, Edupuganti S, Sui J, Morrissey M, McCausland M, Skountzou I, Hornig M, Lipkin WI, Mehta A, Razavi B, Del Rio C, Zheng NY, Lee JH, Huang M, Ali Z, Kaur K, Andrews S, Amara R, Wang Y, Das S, Yewdell J, Subbarao K, Marasco WA, Mulligan MJ, Compans R, Ahmed R, Wilson PC. Broadly cross-reactive antibodies dominate the acute B cell response against novel 2009 H1N1 influenza virus infection. J Exp Med. 2011 Jan 17;208(1):181-93. PMCID: PMC3023136.

Hashem AM, Van Domselaar G, Li C, Wang J, She YM, Cyr TD, Sui J, He R, Marasco WA, Li X. Universal Antibodies against the Highly Conserved Influenza Fusion Peptide Cross-Neutralize Several Subtypes of Influenza A Virus. Biochem Biophys Res Commun. 2010 Dec 10; 403(2):247-51. PMID: 21078301.
Herschhorn A, Marasco WA, Hizi A. Antibodies and Lentiviruses that Specifically Recognize a T-cell Epitope Derived from HIV-1 Nef Protein and Presented by HLA-C. J Immunol. 2010 Dec 15; 185(12):7623-32. PMID: 21076072.

Xu C, Lo A, Yammanuru A, Tallarico AS, Brady K, Murakami A, Barteneva N, Zhu QK, and Marasco WA. Unique biological properties of catalytic domain directed human anti-CAIX antibodies discovered through phage-display technology. PLoS ONE. 2010 March 10;5(3):e9625.  PMID:20224781.

Centeno CJ, Schultz JR, Cheever M, Robinson B, Freeman M, Marasco W. Safety and Complications Reporting on the Re-implantation of Culture-Expanded Mesenchymal Stem Cells using Autologous Platelet Lysate Technique. Curr Stem Cell Res Ther., 2010 Mar;5(1):81-93. PMID: 19951252.

Kuan CT, Srivastava N, McLendon RE, Marasco WA, Zalutsky MR, Bigner DD. Recombinant single-chain variable fragment antibodies against extracellular epitopes of human multidrug resistance protein MRP3 for targeting malignant gliomas. International Journal of Cancer. 2009 Nov. PMID: 19937796.

Gennari F, Lopes L, Verhoeyen E, Marasco WA and Collins MK. Single-chain antibodies that target lentiviral vectors to MHC Class II on antigen-presenting cells. Gene Therapy. Gene Therapy, 2009 Jun;20(6):554-62. PMID: 19260768.

Sultana H, Foellmer HG, Neelakanta G, Oliphant T, Engle M, Ledozet M, Krishnan MJ, Bonafe N, Anthony K, Marasco WA, Kaplan P, Montgomey RR, Diamond MS, Koski RA and Fikrig E.  The fusion loop peptide of the West Nile Virus Envelope Protein is Essential for Pathogenesis and Recognized by a Therapeutic Cross-Reactive Human Monoclonal Antibody. J Immunol. 2009 Jul 1;183(1):650-60. Epub 2009 Jun 17. PMID: 19535627.

Sui J, Hwang WC, Perez S, Wei G, Aird D, Chen L, Santelli E, Stec B, Cadwell G, Ali M, Wan H,  Murakami A, Yammanuru A, Han T, Cox N, Bankston LA, Donis RO, Liddington RC and Marasco WA.  Structural and Functional Bases for Broad-Spectrum Neutralization of Avian and Human Influenza A Viruses.  Nat Struct Mol Biol. 2009 Feb 22. PMCID: PMC2692245.

Nakase K, Cheng J, Zhu Q and Marasco WA. Mechanisms of SHP-1 P2 Promoter Regulation in Hematopoietic cells and its Silencing in HTLV-1 Transformed T-cells.  J Leukocyte Biol. 2008 Oct 23. PMID: 18948549.

Sui, J., D. R. Aird, A. Tamin, A. Murakami, M. Yan, A. Yammanuru, H. Jing, B. Kan, X. Liu, Q. Zhu, Q. Yuan, G. P. Adams, W. J. Bellini, J. Xu, L. J. Anderson, and Marasco WA. Broadening of Neutralization Activity to Directly Block a Dominant Antibody-Driven SARS-Coronavirus Evolution Pathway. PLoS Pathogens 2008. 4:e1000197. PMCID: PMC2572002

Taube R, Zhu Q, Xu C, Diaz-Griffero F, Sui J, Aird D, Dwyer MR and Marasco WA.  Lentivirus Display:  Stable Expression of Human Antibodies on the Surface of Human Cells and Virus Particles.  PLoS ONE. 2008 Sep 11;3(9):e3181. PMCID: PMC2527531.

Huang H, Li W, Sui J, Marasco WA, Choe H, Farzan M. Influenza A virus neuraminidase limits viral superinfection. J. Virol. 2008 May:82(10): 4834-43.  Epub 2008 March 5. PMCID: PMC2346733

Marasco WA and Sui J. The growth and potential of human antiviral monoclonal antibody therapeutics. Nat Biotechnol. 2007 1421-34. PMID: 18066039

Li W, Sui J, Huang IC, Kuhn JH, Radoshitzky SR, Marasco WA, Choe H, Farzan M. The S proteins of human coronavirus NL63 and severe acute respiratory syndrome coronavirus bind overlapping regions of ACE2. Virology. 2007 Oct 25;367(2):367-74. Epub 2007 Jul 12.  PMID: 17631932

Cheng J, Kydd AR, Noonan KM, Murakami A, Tao H, Dwyer M, Xu C, Zhu Q and Marasco WA.  Negative regulation of SH2-Homology-Containing Protein-Tyrosine Phosphatase-1 (Shp-1) P2 Promoter by the HTLV-1 Tax Oncoprotein. Blood, 2007 Sep 15;110(6):2110-20. Epub 2007 May 31. PMCID: PMC1716830

Xu C, Sui J, Tao H, Aird D, Zhu QK and Marasco WA.  Human Anti-CXCR4 Antibodies Undergo VH Replacement, Exhibit Functional V-Region Sulfation and Define CXCR4 Antigenic Heterogeneity. J. Immunol 2007 Aug 15;179(4):2408-18. PMID: 17675502

Kanai R, Kar K, Anthony K, Gould LH, Ledizet M, Fikrig E, Marasco WA, Koski RA and Modis Y.  Crystal structure of West Nile virus envelope glycoprotein reveals viral surface epitopes.  J Virol 2006;80:11000-11008. PMCID: PMC1642136

Bai J, Demirjian A, Sui J, Marasco WA and Callery MP. Histone deacetylase inhibitor trichostatin A and proteasome inhibitor PS-341 synergistically induce apoptosis in pancreatic cancer cells.  Biochem Biophys Res Commun 2006 Oct 6;348(4):1245-1253. PMID: 16904634

Yuan Q-A, Simmons HH, Robinson MK, Russeva M, Marasco WA and Adams GP.  Development of engineered antibodies specific for the M_llerian inhibiting substance type II receptor (MISIIR) - A promising candidate for the targeted therapy of ovarian cancer.  Mol Cancer Ther 2006 5(8):2096-2105. PMID: 16928831

Hwang W, Lin Y, Santelli E, Sui J, Jaroszewski L, Stec B, Farzan M, Marasco WA and Liddington RC.  Structural basis of neutralization by a human anti-severe SARS Spike Protein Antibody, 80R.  J. Biol. Chem. 2006 Nov 10;281(45):34610-34616. PMID: 16954221

Tarnovitski N, Matthews LJ, Sui J, Gershoni JM, Marasco WA. Mapping a Neutralizing Epitope on the SARS Coronavirus Spike Protein: Computational Prediction Based on Affinity-selected Peptides. J Mol Biol 2006;359:190-201. PMID: 16630634

Si Z, Vandegraaff N, O'huigin C, Song B, Yuan W, Xu C, Perron M, Li X, Marasco WA, Engelman A, Dean M, Sodroski J. Evolution of a cytoplasmic tripartite motif (TRIM) protein in cows that restricts retroviral infection. Proc Natl Acad Sci U S A 2006 May 9;103(19):7454-59. PMCID: PMC1464360

Bal HP, Cheng J, Murakami A, Tallarico ASC, Wang W, Zhou D, Vasicek TJ and Marasco WA. GITR overexpression on CD4+CD25+ HTLV-1 transformed cells: detection by massively parallel signature sequencing. Biochem. Biophys. Res. Commun.  2005 Jul 1;332(2):569-84. PMID: 15896717

Marasco WA.  Therapeutic antibody gene transfer.  Nat Biotechnol. 2005 May;23(5):551-2. PMID: 15877069

Song E, Zhu P, Lee S-K, Chowdhury D, Kussman S, Dykxhoorn D, Feng Y, Palliser D, Weiner DB, Shankar P, Marasco WA and Lieberman J.  Antibody-mediated in vivo delivery of short interfering RNAs via cell surface receptors.  Nature Biotechnol. 2005 Jun;23(6):709-17. Epub 2005 May 22. PMID: 15908939.

Gould LH, Sui J, Foellmer H, Oliphant T, Wang T, Ledizet M, Murakami A, Noonan K, Lambeth C, Kar K,  Anderson JF, de Silva AM, Diamond MS, Koski R, Marasco WA, and Fikrig E. Protection and therapeutic capacity of human single-chain-Fc fusion proteins against West Nile virus. J. Virol. 2005 Dec;79(23):14606-14613. PMCID: PMC1287547

Li W, Zhang C, Moore MJ, Kuhn JH, Wing S-K, Vasilieva N, Sui J, Marasco WA, Guan Y, Choe H and Farzan M.  Receptor and Viral Determinants of SARS-Coronavirus Adaptation to Human Antigotension-Converting Enzyme 2.  EMBO J 2005;24(8):1634-43. PMCID: PMC1142572

Bai J, Sui J, Demirjian A, Vollmer CM, Marasco WA and Callery MP.  Predominant Bcl-Xl knockdown disables anti-apoptotic mechanisms:  TRAIL-based triple chemotherapy overcomes chemoresistance in pancreatic cancer cells in vitro. Cancer Res. 2005. Mar 15;65(6):2344-52. PMID: 15781649.

Qin C, Wang J, Wei Q, She M, Marasco WA, Jiang H, Tu X, Zhu H, Ren L, Gao H, Guo L, Huang L, Yang R, Cong Z, Guo L, Wang Y, Liu Y, Sun Y, Duan S, Qu J, Chen L, Tong W, Ruan L, Peimao L, Zhang H, Zhang J, Zhang H, Liu D, Liu Q, He W, and Hong T.  An animal model of SARS produced by infection of Macaca mulatta with SARS coronavirus.  J. Pathol. 2005. Jul;206(3):251-9. PMID: 15892035

Sui J, Li W, Roberts A, Matthews LJ, Murakami A, Vogel L, Wong SK, Subbarao K, Farzan M, and Marasco WA.  Evaluation of Human mAb 80R for Immunoprophylaxis of SARS by an Animal Study, Epitope Mapping and Analysis of Spike Variants.  J.Virol. 2005 May;79(10):5900-6. PMCID: PMC1091676

Masiero S, Del Vecchio C, Gavioli R, Mattiuzzo G, Cusi MG, Micheli L, Gennari F, Siccardi A, Marasco WA, Palu G, Parolin C. T-cell engineering by a chimeric T-cell receptor with antibody-type specificity for the HIV-1 gp120. Gene Ther 2005 Feb;12:299-310. PMID: 15496956.

Beyer F, Doebis C, Busch A, Ritter T, Mhashilkar A, Marasco WA, Laube H, Volk HD, Seifert M.  Decline of Surface MHC I by Adenoviral Gene Transfer of Anti-MHC I Intrabodies in Human Endothelial Cells - New Perspectives for the Generation of Universal Donor Cells for Tissue Transplantation.  J Gene Med. 2004 Jun;6(6):616-23. PMID: 15170732

Zhu Q, Ricardo RR, Zhang L, Ogueta SB, Agrawal RS, Dzau VJ, and Marasco WA.  Development of constitutive and inducible self-inactivating lentiviral vectors and their application in cardiovascular gene transfer.  Gene Ther Mol Biol., 2004; (8): 91-102. pdf

Sui J, Li W, Murakami A, Tamin A, Matthews LJ, Wong SK, Moore MJ, Tallarico AS, Olurinde M, Choe H, Anderson LJ, Bellini WJ, Farzan M, and Marasco WA.  Potent Neutralization of (SARS) Coronavirus by a Human mAb to S1 protein that blocks receptor association.  Proc Natl Acad Sci U S A. 2004 Feb 24;101(8):2536-41. PMCID: PMC356985

Gennari F, Mehta S, Wang Y, Tallarico A, Palu G, and Marasco WA.  Direct Phage to Intrabody Screening:  Demonstration by Isolation of Cytosolic Intrabodies Against the TES1 Site of Epstein Barr Virus Latent Membrane Protein 1 (LMP1) that Block NF-kappaB Transactivation.  Journal of Molecular Biology, 2004; 335: 193 - 207. PMID: 14659750

Cheng, J, Zhang, D, Zhou, C, and Marasco, WA.  Down-Regulation of SHP1 and Up-Regulation of Negative Regulators of JAK/STAT Signaling in HTLV-1 Transformed Cell Lines and Freshly Transformed Human Peripheral Blood CD4+ T-cells.  Leuk Res. 2004 Jan;28(1):71-82. PMID: 14630083

Busch, A, Marasco, WA, Doebis, C, Volk, H-D, Seifert, M.  MHC Class I Manipulation on Cell Surfaces by Gene Transfer of Anti-MHC Class I Intrabodies - A tool for Decreased Immunogenicity of allogeneic tissue and cell transplants. Methods, 2004; 34(2):240-249. PMID: 15312677

Sui J, Bai J, St Clair Tallarico A, Xu C, Marasco WA. Identification of CD4 and transferrin receptor antibodies by CXCR4 antibody-guided Pathfinder selection. Eur J Biochem 2003 Nov;270:4497-506. PMID: 14622278

Zhang D, Murakami A, Johnson RP, Sui J, Cheng J, and Marasco WA. Optimization of ex vivo activation and expansion of macaque primary CD4-enriched peripheral blood mononuclear cells for use in anti-HIV immunotherapy and gene therapy strategies. J Acquir Immune Defic Syndr., 2003 Mar:1;32(3):245-54. PMID: 12626883.

Bai J, Sui J, Zhu R-Y, Tallarico AStC, Gennari F, Zhang D, and Marasco WA.  Inhibition of Tat-Mediated Transactivation and HIV-1 Replication by Human Anti-hCyclin T1 Intrabodies.  J. Biol. Chem., 2003;278:1433-1442. PMID: 12401780

Sui J, and Marasco WA.  Evidence Against Ebola Virus sGP Binding to Human Neutrophils by a Specific Receptor.  Virology, 2002;303:9-14. PMID: 12482654

Mhashilkar AM, Doebis C, Seifert M, Busch A, Zani C, Soo Hoo J, Nagy M, Ritter T, Hans-Dieter V, and Marasco WA.  Intrabody-Mediated Phenotypic Knockout of Major Histocompatibility Complex Class 1 Expression in Human and Monkey Cell Lines and in Primary Human Keratinocytes.  Gene Ther 2002;9:307-319. PMID: 11938450

Li X, Josef J, and Marasco WA.  HIV-1 Tat can substantially enhance the capacity of NIK to induce IkappaB degradation.  Biochem Biophys Res Comm 2001; 286:587-94. PMID: 11511100

Li X, Stuckert P, Bosch I, Marks JD, and Marasco WA.  Single-chain antibody mediated-gene delivery into ErbB2 positive human breast cancer cells.  Cancer Gene Ther 2001;8:555-65. PMID: 11571533

Ogueta SB, Yao F, and Marasco WA.  Design and in vitro characterization of a single regulatory module for efficient control of gene expression in both plasmid DNA and a self-inactivating lentiviral vector.  Mol Med 2001;7:569-71. PMCID: PMC1950063

Li X, Multon M-C, Henin Y, Schweighoffer F, Venot C, LaVecchio J, Josef J, Stuckert P, Mhashilkar A, Tocque B and Marasco WA.  Upregulation of the apoptosis associated protein Grb3-3 in HIV-1-infected human CD4+ lymphocytes. Biochem Biophys Res Comm 2000;276:362-70. PMID: 11006130

Li X, Multon M-C, Henin Y, Schweighoffer F, Venot C, Josef J, Zhou C, LaVecchio J, Stuckert P, Raab M, Mhashilkar A, Tocque B and Marasco WA.  Grb3-3 is up-regulated in HIV-1 infected T-cells and can potentiate cell activation through NFATc. J Biol Chem 2000;275:30925-33. PMID: 10906142

Mirzabekov T, Kontos H, Farzan M, Marasco W and Sodroski J.  Paramagnetic proteoliposomes containing a pure, native, and oriented seven-transmembrane segment protein, CCR5.  Nat Biotechnol 2000;18:649-54. PMID: 10835604

Marasco W, LaVecchio J and Winkler A.  Human anti-HIV-1 Tat sFv intrabodies for gene therapy of advanced HIV-1-infection and AIDS.  J Immunol Meth 1999;231: 223-38. PMID: 10648940

Zhu Q, Zeng C, Huhalov A, Yao J, Turi TG, Danley D, Hynes T, Cong Y, DiMattia D, Kennedy S, Daumy G, Schaeffer E, Marasco WA and Huston JS.  Extended half-life and elevated steady-state level of a single-chain Fv intrabody are critical for specific intracellular retargeting of its antigen, Capase-7.  J Immunol Meth 1999;231:207-22. PMID: 10648939

Mhashilkar AM, LaVecchio J, Eberhardt B, Brooks J, Boisot S, Dove JH, Pumphrey C, Li X, Weissmahr RN, Ring DB, Ramstedt U and Marasco WA.  Inhibition of human immunodeficiency virus type 1 replication in vitro in acutely and persistently infected human CD4+ mononuclear cells expressing murine and humanized anti-HIV-1 tat sFv intrabodies.  Hum Gene Ther 1999;10:1453-67. PMID: 10395371

Poznansky MC, La Vecchio J, Silva-Arietta S, Porter-Brooks J, Brody K, Olszak IT, Adams GB, Ramstedt U, Marasco WA, Scadden DT.  Inhibition of human immunodeficiency virus replication and growth advantage of CD4+ T-cells and monocytes derived from CD34+ cells transduced with an intracellular antibody directed against human immunodeficiency virus type 1 Tat. Hum Gene Ther 1999 Oct 10;10:2505-14. PMID: 10543615

Marasco WA, Chen SY, Richardson JH, Ramstedt U, Jones SD.  Clinical Protocol: Intracellular antibodies against HIV-1 envelope protein for AIDS gene therapy.  Hum Gene Ther 1998;9:1627-42. PMID: 9694161

Richardson JH, Hofmann W, Sodroski JG and Marasco WA.  Intrabody-mediated knockout of the high-affinity IL-2 receptor in primary human T-cells using a bicistronic lentivirus vector.  Gene Ther 1998;5:635-44. PMID: 9797868

Poznansky MC, Foxall R, Mhashilkar A, Coker R, Jones S, Ramstedt U, Marasco W. Inhibition of HIV replication and growth advantage of CD4+ T-cells from HIV-infected individuals which express intracellular antibodies against HIV-1 gp120 or Tat.  Hum Gene Ther 1998;9:487-96. PMID: 9525310

Vallat A-V, DeGirolami U, He J, Mhashilkar A, Marasco W, Shi B, Gray F, Bell J, Keohane C, Smith TW and Gabuzda D.  Localization of HIV-1 coreceptors CCR5 and CXCR4 in the brain of children with AIDS.  Am J Path 1998;152:167-78. PMCID: PMC1858124

Richardson JH, Waldmann TA, Sodroski JG, Marasco WA. Inducible knockout of the interleukin-2 receptor alpha chain: expression of the high-affinity IL-2 receptor is not required for the in vitro growth of HTLV-I-transformed cell lines. Virology 1997 Oct 27;237:209-16. PMID: 9356333

Mhashilkar AM, Biswas DK, Pardee AB and Marasco WA. Inhibition of HIV-1 replication in-vitro by a novel combination of anti-tat single chain intrabodies and NF-kappaB antagonists.  J Virol  1997;71:6486-94. PMCID: PMC191923

Marasco WA.  Intrabodies: Turning the Humoral Immune System Outside In for Intracellular Immunization.  Gene Therapy 1997;4:11-15. PMID: 9068790

Levin R, Mhashilkar AM, Dorfman T, Bukovsky A, Zani C, Bagley J, Hinkula J, Niedrig M, Albert J, Wahren B, Göttlinger H and Marasco WA.  Inhibition of early and late events of the HIV-1 replication cycle by cytoplasmic Fab intrabodies against the matrix protein, p17.  Mol Med 1997;2:96-110. PMCID: PMC2230055

Chen J, Yang Q, Yang A-G, Marasco WA and Chen S-Y.  Intra- and extracellular immunization against HIV-1 infection with lymphocytes transduced with an AAV vector expressing a human anti-gp120 antibody.  Hum Gene Ther 1996;7:1515-25. PMID: 8864752

Richardson JH and Marasco WA.  Intracellular antibodies:  Development and therapeutic potential.  Trends in Biotech 1995;13(8):306-10. PMID: 7662306

Marasco WA.  Intracellular antibodies (intrabodies) as research reagents and therapeutic molecules for gene therapy.  Immunotechnology 1995;1:1-19. PMID: 9373329

Richardson JH, Sodroski JG, Waldman TA, and Marasco WA.  Phenotypic knockout of the high-affinity human interleukin 2 receptor by intracellular single-chain antibodies against the _ subunit of the receptor.  Proc Natl Acad Sci USA 1995;92:3137-41. PMCID: PMC42120

Mhashilkar AM, Bagley J, Chen S-Y, Szilvay AM, Helland DG, and Marasco WA.  Inhibition of HIV-1 tat-mediated LTR transduction and HIV-1 infection by anti-tat single chain intrabodies.  EMBO J 1995;14(7):1542-51. PMCID: PMC398241

Chen S-Y, Zani C, Khouri YF, and Marasco WA.  Design of a genetic immunotoxin to eliminate toxin immunogenicity.  Gene Therapy 1995;2:116-23. PMID: 7719928.

Khouri YF, McIntosh K, Cavacini L, Posner M, Pagano M, Tuomala R, and Marasco WA.  Vertical transmission of HIV-1:  Correlation with maternal viral load and plasma levels of CD4 binding site anti-gp120 antibodies.  J Clin Invest 1995;95:732-7. PMID: 7860754.

Bagley J, Dillon PJ, Rosen C, Robinson J, Sodroski J, and Marasco WA.  Structural characterization of broadly neutralizing human monoclonal antibodies against the CD4 binding site of HIV-1 gp120.  Mol Immunol 1994;31:1149-60. PMID: 7935503.

Marasco WA, Szilvay AM, Kalland KH, Helland DG, Reyes HM, Walter RJ. Spatial association of HIV-1 tat protein and the nucleolar transport protein B23 in stably transfected Jurkat T-cells. Arch Virol 1994;139:133-54. PMID: 7826206.

Chen S-Y, Khouri YF, Bagley J, and Marasco WA.  Combined intra- and extracellular immunization against HIV-1 infection using a human anti-gp120 antibody.  Proc Natl Acad Sci USA 1994;91:5932-6. PMCID: PMC44111

Chen S-Y, Bagley J, and Marasco WA.  Intracellular antibodies as a new class of therapeutic molecules for gene therapy.  Human Gene Therapy 1994;5:595-601. PMID: 7914435

Marasco WA, Haseltine WA, and Chen S-Y.  Design, intracellular expression, and activity of a human anti-HIV-1 gp120 single chain antibody.  Proc Natl Acad Sci USA 1993;90:7889-93. PMID: 8356098.

Marasco WA, Bagley J, Zani C, Posner M, Cavacini L, Haseltine WA, and Sodroski JG. Characterization of the cDNA of a broadly reactive neutralizing human anti-gp120 monoclonal antibody.  J Clin Invest 1992;90:1467-78. PMID: 1401079.

Garcia LW, Hemphill RB, Marasco WA, and Ciano PS.  Acquired immunodeficiency syndrome with disseminated toxoplasmosis presenting as an acute pulmonary and gastrointestinal illness.  Arch Path Lab 1991;115:459-63. PMID: 2021313.

Bradley SF, Gordon JJ, Baugartner DD, Marasco WA, and Kauffman CA.  Group C streptococcal bacteremia:  An analysis of 88 cases.  Rev Infectious Dis 1991;13:270-80. PMID: 2041960.

Sannomiya P, Craig RA, Clewell DB, Suzuki A, Fujino M, Till GO, and Marasco WA. Characterization of a new class of Enterococcus faecalis derived neutrophil chemotactic peptides, the sex pheromones.  Proc Natl Acad Sci USA 1990;87:66-70. PMID: 2104986.

Janeczek AH, Marasco WA, Van Alten PJ, and Walter RJ.  Autoradiographic analysis of formyl peptide chemoattractant binding, uptake and intracellular processing by neutrophils.  J Cell Sci 1989;94:155-68. PMID: 2515197.

Marasco WA, Lester S, and Parsonnet J.  Unusual presentation of cat scratch disease in a patient positive for antibody to the human immunodeficiency virus.  Rev Inf Dis 1989;11:793-803. PMID: 2814165.

Feltner DE and Marasco WA.  Regulation of formyl-peptide receptor binding to rabbit neutrophil plasma membranes:  Use of monovalent cations, guanine nucleotides and bacterial toxins to discriminate among different states of the receptor.  J Immunol 1989;142:3963-70. PMID: 2715641.

Atkinson YH, Lopez AF, Marasco WA, Lucas C, Wong GG, Burns GF, and Vadas MA. Recombinant human granulocyte-macrophage colony-stimulating factor (rH GM-CSF) regulates the expression of f Met-Leu-Phe receptors on human neutrophils.  Immunology  1988;64:519-25. PMID: 2842255.

Atkinson YH, Marasco WA, Lopez AF, and Vadas MA.  Recombinant human tumor necrosis factor-alpha(rH TNF-alpha).  Regulation of N-formylmethionylleucylphenylalanine receptor expression and function on human neutrophils.  J Clin Invest 1988;81:759-65. PMID: 2830314

Marasco WA, Gikas PW, Azziz-Baumgartner R, Huzy R, Eldredge CJ, and Stross J.  Ibuprofen associated renal dysfunction:  Pathophysiologic mechanisms of acute renal failure, hyperkalemia, tubular necrosis and proteinuria.  Arch Intern Med 1987;147: 2107-16. PMID: 3689062.

Walter RJ and Marasco WA.  Direct visualization of formylpeptide receptor binding on rounded and polarized human neutrophils:  Cellular and receptor heterogeneity.  J Leukocyte Biol  1987;41:377-91. PMID: 3553401.

Feltner DE, Smith RH, and Marasco WA.  Characterization of the plasma membrane bound GTPase activity from rabbit neutrophils:  I. Evidence for an Ni-like protein coupled to the formyl peptide, C5a and leukotriene B4 chemotaxis receptors.  J Immunol 1986;137:1961-70. PMID: 3018082.

Duque RE, Fantone JC, Kramer C, Marasco WA, and Phan SH.  Inhibition of neutrophil activation by p-bromophenacyl bromide and its effects on phospholipase A2. Brit J Pharm  1986;88:463-72. PMID: 3015312.

Marasco WA, Becker KM, Feltner DE, Brown CS, Nairn R, and Ward PA.  Covalent affinity labeling, detergent solubilization, and fluid phase characterization of the rabbit neutrophil formyl peptide chemotaxis receptor.  Biochemistry 1985;24:2227-36. PMID: 3995012.

Marasco WA, Ward PA, Feltner DE, and Varani J.  Chemotactic factor binding by metastatic tumor cells:  evidence for a formyl-peptide receptor on a non-myelogenous cell.  J Cell Sci  1985;73:121-34. PMID: 4019589.

Marasco WA, Feltner DE, and Ward PA.  Formyl peptide chemotaxis receptors on the rat neutrophil.  Experimental evidence for negative cooperativity.  J Cell Biochem 1985; 27:359-75. PMID: 2987275.

Walter RJ, Marasco WA. Localization of chemotactic peptide receptors on rabbit neutrophils. Exp Cell Res 1984 Oct;154:613-8. PMID: 6479242.

Fantone JC, Marasco WA, Elgas LJ, and Ward PA.  Stimulus specificity of prostaglandin inhibition of rabbit polymor phonuclear leukocyte lysosomal enzyme release and superoxide anion production.  Am J Pathol 1984;115:9-16. PMID: 6324595.

Marasco WA, Phan SH, Krutzsch H, Showell HJ, Feltner DE, Nairn R, Becker EL, and Ward PA.  Purification and identification of formyl-methionyl-leucyl-phenylalanine as the major peptide neutrophil chemotactic factor produced by Escherichia coli.  J Biol Chem 1984;259:5430-9. PMID: 6371005.

Kunkel SL, Manderino GL, Marasco WA, Kaercher K, Hirata AA, and Ward PA.  A specific enzyme-linked immunosorbent assay (ELISA) for the determination of human C5a antigen.  J Immunol Meth 1983;62:305-14. PMID: 6193199.

Fantone JC, Marasco WA, Elgas LJ, and Ward PA.  Anti-inflammatory effects of prostaglandin E1:  In vivo modulation of the formyl peptide chemotactic receptor on the rat neutrophil.  J Immunol 1983;130:1495-7. PMID: 6300229.

Marasco WA, Fantone JC, Freer RJ, and Ward PA.  Characterization of the rat neutrophil formyl peptide chemotaxis receptor.  Am J Pathol 1983;111:273-81. PMID: 6305198.

Marasco WA, Fantone JC, and Ward PA.  Spasmogenic activity of chemotactic N-formulated oligopeptides:  Identity of structure-function relationships for chemotactic and spasmogenic activities.  Proc Natl Acad Sci USA 1982;79:7470-3. PMID: 6296835.

Marasco WA and Becker EL.  Anti-idiotype as antibody against the formyl peptide chemotaxis receptor of the neutrophil.  J Immunol 1982;128:963-8. PMID: 7054297.

Marasco WA, Showell HJ, Freer RJ, and Becker EL.  Anti-f Met-Leu-Phe.  Similarities in fine specificity with the formyl peptide chemotaxis receptor of the neutrophil.  J Immunol 1982;128:956-62. PMID: 7033385.

Marasco WA, Showell HJ, and Becker EL.  Substance P binds to the formyl peptide chemotaxis receptor on the rabbit neutrophil.  Biochem Biophys Res Comm 1981;99: 1065-72. PMID: 6167265.

Marasco WA, Becker EL, and Oliver JM.  The Ionic Basis of Chemotaxis.  Separate cation requirements for neutrophil orientation and locomotion in a gradient of chemotactic peptide.  Am J Pathol 1980;98:749-68. PMID: 6767408.